Click this text to review the details of the improved versions of the Mouse Th Cytokine Panels.T helper (Th) cells play important roles in regulating immune responses. They secrete cytokines to stimulate various effector cells, such as cytotoxic T cells, B cells and macrophages. These cytokines are also involved in inflammatory and autoimmune diseases. Accurate measurement of Th cytokine expression is critical for in-depth understanding of immune responses and many disease processes.

The LEGENDplex™ Mouse Th Cytokine Panel is a bead-based multiplex assay panel, using fluorescence-LEGENDplex™encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 Mouse cytokines, including IL-2, 4, 5, 6, 9, 10, 13, 17A, 17F, 21, 22, IFN-γ and TNF-α, which are collectively secreted by Th1, Th2, Th9, Th17, Th22 and T follicular cells. This assay panel provides higher sensitivities and broader dynamic range than traditional ELISA methods. The panel has been validated for use on serum and cell culture supernatant samples.

The LEGENDplex™ Mouse Th Cytokine Panel is designed to allow flexible customization within the panel. It can also be divided into sub-panels, such as:

LEGENDplex™ Mouse Th Panel (13-Plex)LEGENDplex™ Mouse Th1 Panel (5-Plex)LEGENDplex™ Mouse Th2 Panel (6-Plex)LEGENDplex™ Mouse Th17 Panel (8-Plex)LEGENDplex™ Mouse Th1/Th2 Panel (8-Plex)LEGENDplex™ Mouse Th9 Panel (3-Plex)LEGENDplex™ Mouse Th22 Panel (4-Plex)LEGENDplex™ Mouse Tfh Panel (4-Plex)

Please refer to the target selection table for panel-specific target information (Table 1). Please visit www.biolegend.com/legendplex for more information on panel design and how to mix and match within the panel.